Rabbit Anti-Mouse PHOX2B (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Host Species | Rabbit |
| Accession Number | Q99453 |
| Gene Id | 8929 |
| Gene Symbols | PHOX2B |
| Protein Name / Synonyms | PHOX2B (Center) |
| Clonality | Polyclonal |
| Preparation | This PHOX2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PHOX2B. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The DNA-associated protein encoded by this gene is a member of the paired family of homeobox proteins localized to the nucleus. The protein functions as a transcription factor involved in the development of several major noradrenergic neuron populations and the determination of neurotransmitter phenotype. The gene product is linked to enhancement of second messenger-mediated activation of the dopamine beta-hydroylase, c-fos promoters and several enhancers, including cyclic amp-response element and serum-response element. [provided by RefSeq].
Specificity
Predicted Reactivity
Human, Rat
Other Names
PHOX2B; PMX2B; Paired mesoderm homeobox protein 2B; Neuroblastoma Phox; PHOX2B homeodomain protein; Paired-like homeobox 2B
NCBI Accession #
NP_003915.2
Other Accession #
O35690; Q62782; Q62066; O14813; NP_003915.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
31621
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent image of U251 cell stained with PHOX2B Antibody (Center)(Cat# 102-11055).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PHOX2B primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°).PHOX2B immunoreactivity is localized to Nucleus significantly. |
 | PHOX2B Antibody (Center) (Cat# 102-11055) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the PHOX2B antibody detected the PHOX2B protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.