Ly-6A/E is also known as, stem cell antigen 1 (Sca-1). Ly-6A/E is a 18kD phosphatidylinositol-anchored protein that is expressed on hematopoietic stem cells (HSC) in mice with both Ly-6 haplotypes. Murine HSC are found among Lin-Sca-1+ cells in the adult bone marrow and fetal liver. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, while strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. Combined with lineage cocktails, Sca-1 is an important marker for phenotyping bone marrow stem cells and progenitor cells.

Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.

Stored at 2 °C - 8 °C, protected from prolonged light exposure. DO NOT FREEZE.