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Anti-Mouse Sca-1 Antibody, PE-Cy7
Catalog #: 135-23044
Mouse Sca-1 Antibody [clone: E13161-7], monoclonal rat IgG2a kappa. Validated for flow cytometry.
Product Description
Specifications
| Size | 25 µg, 100 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Host Species | Rat |
| Protein Name / Synonyms | Sca-1, Ly-6A/E |
| Clonality | Monoclonal |
| Clone | E13161-7 |
| Conjugation | PE-Cy7 |
| Preparation | The antibody was purified by affinity chromatography. |
| Isotype | IgG2a Kappa |
| Format | Liquid |
| Concentration (lot specific) | 0.5 mg/ml |
| Buffers | PBS, pH 7.2-7.4, 0.2% BSA, 0.1% sodium azide |
| Recommended Applications | Flow Cytometry |
| Research Area | Cardiovascular Disease |
| Excitation Laser | Green Laser, 565 nm |
| Maximum Emission Wavelength | 778 nm |
| Shipping Type | Blue ice |
| Storage | 4°C |
Description
Product Features
- Serum-free media used for cell culture of hybridomas to minimize bovine antibody contamination
- Gentle elution at pH 4.0 to minimize antibody degradation
- Protein A, G & L affinity purification
- High purity
Description
Ly-6A/E is also known as, stem cell antigen 1 (Sca-1). Ly-6A/E is a 18kD phosphatidylinositol-anchored protein that is expressed on hematopoietic stem cells (HSC) in mice with both Ly-6 haplotypes. Murine HSC are found among Lin-Sca-1+ cells in the adult bone marrow and fetal liver. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, C57L, C58, DBA/2, PL, SJL, SWR, 129), Ly-6A/E is also expressed on distinct subpopulations of bone marrow and peripheral B lymphocytes, myeloid cells, and thymic and peripheral T lymphocytes, while strains with the Ly-6.1 haplotype (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E+ resting peripheral lymphocytes, whereas activation of T cells from mice of both Ly-6 haplotypes leads to strong expression of the Sca-1 antigen. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. Combined with lineage cocktails, Sca-1 is an important marker for phenotyping bone marrow stem cells and progenitor cells.
Recommended Usage
Each lot of antibody has been tested by flow cytometry staining. We suggest use ≤ 0.5 µg per million cells in 100 µl volume of staining buffer. Researchers are recommended to further titrate this antibody at the optimal concentration for each specific application.
Storage / Stability
Store at 2 °C ~ 8 °C, protected from prolonged light exposure. DO NOT FREEZE.