Mouse Anti-Mouse TSC2 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Mouse |
| Accession Number | P49815 |
| Gene Id | 7249 |
| Gene Symbols | TSC2 |
| Protein Name / Synonyms | TSC2 |
| Clonality | Monoclonal |
| Preparation | This TSC2 monoclonal antibody is generated from mouse immunized with TSC2 recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cardiovascular Disease, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
Specificity
Other Names
TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein
NCBI Accession #
NP_000539.2;NP_001070651.1;NP_001107854.1
Other Accession #
NP_000539.2; NP_001070651.1
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
200608
Recommended Dilutions
IF: 1:10-50; WB: 1:100
 | Fluorescent image of Hela cell stained with TSC2 Antibody(Cat# 102-10150/SG110509AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSC2 immunoreactivity is localized to Microtubules significantly. |
 | TSC2 Antibody (Cat# 102-10150) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
