Synonyms
MAP2K1IP1 antibody, MAPBP antibody, MAPKSP1 antibody, MP1 antibody, Ragulator3 antibody, LAMTOR3 antibody, mitogen-activated protein kinase kinase 1 interacting protein 1 antibody, mitogen-activated protein kinase scaffold protein 1 antibody, MEK binding partner 1 antibody, ragulator complex protein LAMTOR3 antibody, late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody, MAPK scaffold protein 1 antibody, "late endosomal/lysosomal adaptor, MAPK and MTOR activator 3 antibody"
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
14 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 124 of MP1 (Uniprot ID# Q9UHA4)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MP1(107-10947) antibody at 1:500 dilution.

MP1 antibody detects MP1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg Raw264.7 whole cell lysate/extract
C. 30 µg C2C12 whole cell lysate/extract
15 % SDS-PAGE
MP1 antibody (107-10947) dilution: 1:1000

MP1 antibody detects MP1 protein by Western blot analysis.
A. 30 µg Rat2 whole cell lysate/extract
15 % SDS-PAGE
MP1 antibody (107-10947) dilution: 1:1000

Sample (30 ug of whole cell lysate)
A: 293T
15% SDS PAGE
107-10947 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.