MPI antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Gene Id | 4351 |
| Protein Name / Synonyms | MPI |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CDG1B antibody, FLJ39201 antibody, PMI antibody, PMI1 antibody, MPI antibody, mannose-6- phosphate isomerase antibody, phosphohexomutase antibody, mannose-6-phosphate isomerase antibody, phosphomannose isomerase 1 antibody, mannose phosphate isomerase antibody
Specificity
Species Reactivity
Human, Rat
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
47 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 150 and 423 of MPI (Uniprot ID# P34949)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
MPI antibody detects MPI protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
MPI antibody (107-10398) dilution: 1:1000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
MPI antibody (107-10398) dilution: 1:1000
Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI(107-10398) antibody at 1:100 dilution.
Sample(30µg whole cell lysate)
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
10% SDS PAGE
107-10398 diluted at 1:1000
A: 293T
B: A431
C: H1299
D: HeLa S3
E: Hep G2
F: MOLT4
G: Raji
10% SDS PAGE
107-10398 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.