MRCK alpha antibody, 2 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 8476 |
| Protein Name / Synonyms | MRCK alpha |
| Concentration (lot specific) | 2 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
DKFZp686L1738 antibody, DKFZp686P1738 antibody, FLJ23347 antibody, KIAA0451 antibody, MRCK antibody, MRCKA antibody, PK428 antibody, CDC42BPA antibody, DMPK-like alpha antibody, ser-thr protein kinase PK428 antibody, ser-thr protein kinase related to the myotonic dystrophy protein kinase antibody, myotonic dystrophy protein kinase-like alpha antibody, CDC42 binidng protein kinase beta antibody, myotonic dystrophy kinase-related CDC42-binding protein kinase alpha antibody, MRCK alpha antibody, CDC42-binding protein kinase alpha (DMPK-like) antibody, serine/threonine-protein kinase MRCK alpha antibody, myotonic dystrophy kinase-related CDC42-binding kinase alpha antibody, CDC42 binding protein kinase alpha (DMPK-like) antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
197 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 373 and 564 of MRCK alpha
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha(107-10346) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha(107-10346) antibody at 1:100 dilution.
MRCK alpha antibody detects CDC42BPA protein by Western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
5 % SDS-PAGE
MRCK alpha antibody (107-10346) dilution: 1:1000
A. 30 µg U87-MG whole cell lysate/extract
5 % SDS-PAGE
MRCK alpha antibody (107-10346) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.