Anti-MRPL28 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q13084
Gene Id	10573
Gene Symbols	MRPL28
Protein Name / Synonyms	MRPL28 (N-term)
Clonality	Polyclonal
Preparation	This MRPL28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-55 amino acids from the N-terminal region of human MRPL28.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion.

Specificity

Other Names

MRPL28; MAAT1; 39S ribosomal protein L28, mitochondrial; Melanoma antigen p15; Melanoma-associated antigen recognized by T-lymphocytes

NCBI Accession #

NP_006419.2

Other Accession #

NP_006419.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

30157

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

MRPL28 Antibody (N-term) (Cat# 102-14160) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MRPL28 antibody detected the MRPL28 protein (arrow).

Fluorescent confocal image of A431 cell stained with MRPL28 Antibody (N-term)(Cat# 102-14160).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MRPL28 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MRPL28 immunoreactivity is localized to Mitochondria significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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