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Anti-MRSA Antibody, Biotinylated
Catalog #: 130-10151B-50
Mouse anti-MRSA Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Staphylococcus spp |
| Host Species | Mouse |
| Accession Number | NP_359415.1 |
| Clonality | Monoclonal |
| Immunogen | Immunogen was PBP2a recombinant protein derived from MRSA bacteria. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with PBP2a. The IgM fraction of tissue culture supernatant was purified by Protein G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1: 80, 000), Western Blotting (recommended work dilution= 1:1000) , (see image) Western Blot: First Antibody: 1ug/ml (Mouse anti-PBP2a (1X1000)), Second Antibody: 5000X (HRP-Goat Anti-Mouse IgM) M: Protein Marker Lane 1: Recombinant PBP2a 1ug Lane 2: MSSA lysate 15ul |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Infectious Disease, Antimicrobial Resistance |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen responsible for serious hospital infections worldwide. These bacteria are resistant to all beta-lactam antibiotics due to the production of an additional penicillin binding protein, the PBP2a (about 75kDa) encoded by the mecA gene, which shows low affinity for this class of antibiotics. According to the resistant and sensitive ability of the bacteria to antibiotics, two main groups are classified: MRSA and MSSA (Methicillinsensitive Staphylococcus aureus).
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody was selected for its ability to specifically detect PBP2a from MRSA. The antibody has ability to distinguish both MRSA and MSSA in tested assays; in Western Blot analysis, this antibody did not produce a band for any bacterial cell lysates from MSSA. This antibody showed no cross-reactivity with other tested bacterial proteins.
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Expiration:
12 months from the date of shipment when stored properly.