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Anti-MSLN Antibody
Catalog #: 130-10064
Rabbit Anti-Human MSLN Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q13421 |
| Gene Id | 10232 |
| Gene Symbols | MSLN MPF |
| Clonality | Polyclonal |
| Immunogen | The Immunogen was recombinant protein from Human MSLN. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Recommended Applications | ELISA (recommended work dilution= 1:160, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MSLN. The membrane blot was probed with antiMSLN primary antibody (1 µg/ml). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular MSLN from human and mouse cells (34 kDa). |
| Antigen Catalog Number | 230-00043 |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Mesothelin (MSLN) may play an important role in cellular adhesion. It presents on normal mesothelial cells lining the pleura, peritoneum and pericardium. Since mesothelin is overexpressed in some cancers such as mesothelioma and ovarian and pancreatic adenocarcinoma, it could be a potential tumor biomarker. Mesothelin is immunogenic, and it also may be the potential antigenic target of a therapeutic cancer vaccine.
Specificity
This antibody binds to Human MSLN. Cross reactivity with mouse and rat MSLN was not tested.
Storage
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilizedantibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Related Products
Recombinant Human Mesothelin / Megakaryocyte-Potentiating Factor (catalog#: 230-00043).
References
- Onda M, et al.. (2006) Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma. Clin Cancer Res. 12:4225-31.
- Ho M, et al.. (2005) Humoral immune response to mesothelin in mesothelioma and ovarian cancer patients. Clin Cancer Res. 11(10):3814-20.
Expiration:
12 months from the date of shipment when stored properly.