Rabbit anti-MSLN Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13421 |
| Gene Id | 10232 |
| Gene Symbols | MSLN MPF |
| Clonality | Polyclonal |
| Immunogen | The Immunogen was recombinant protein from Human MSLN. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1:160, 000), Western Blotting (recommended work dilution= 1: 1, 000), (see image) Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein MSLN. The membrane blot was probed with antiMSLN primary antibody (1 µg/ml). Proteins were visualized using a Donkey Anti-Rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate cellular MSLN from human and mouse cells (34 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Cancer |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Mesothelin (MSLN) may play an important role in cellular adhesion. It presents on normal mesothelial cells lining the pleura, peritoneum and pericardium. Since mesothelin is overexpressed in some cancers such as mesothelioma and ovarian and pancreatic adenocarcinoma, it could be a potential tumor biomarker. Mesothelin is immunogenic, and it also may be the potential antigenic target of a therapeutic cancer vaccine.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to Human MSLN. Cross reactivity with mouse and rat MSLN was not tested.
Related Products
Recombinant Human Mesothelin / Megakaryocyte-Potentiating Factor (catalog#: 230-00043).
References
- Onda M, et al.. (2006) Megakaryocyte potentiation factor cleaved from mesothelin precursor is a useful tumor marker in the serum of patients with mesothelioma. Clin Cancer Res. 12:4225-31.
- Ho M, et al.. (2005) Humoral immune response to mesothelin in mesothelioma and ovarian cancer patients. Clin Cancer Res. 11(10):3814-20.
Expiration:
12 months from the date of shipment when stored properly.
