Rabbit Anti-Human mTOR (FRAP1) (S2481) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P42345 |
| Gene Id | 2475 |
| Gene Symbols | MTOR |
| Protein Name / Synonyms | mTOR (FRAP1) (S2481) |
| Clonality | Polyclonal |
| Preparation | This mTOR (FRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2459-2488 amino acids from human mTOR (FRAP1). |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Research Area | Cardiovascular Disease, Inflammation, mTOR Signaling, Autophagy |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
FRAP1 belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. FRAP1 is a part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1; Serine/threonine-protein kinase mTOR; FK506-binding protein 12-rapamycin complex-associated protein 1; FKBP12-rapamycin complex-associated protein; Mammalian target of rapamycin; Mechanistic target of rapamycin; Rapamycin and FKBP12 target 1; Rapamycin target protein 1
NCBI Accession #
NP_004949.1
Other Accession #
P42346; Q9JLN9
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
288892
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50
 | Western blot analysis of FRAP1 (arrow) using rabbit polyclonal FRAP1 Antibody (S2481) (Cat# 102-16716). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FRAP1 gene (Lane 2) (Origene Technologies). |
 | Formalin-fixed and paraffin-embedded human testis tissue reacted with FRAP1-pS2481, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of mTOR (FRAP1) Antibody (S2481)(Cat# 102-16716) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.