Anti-MVP/LRP Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	9961
Protein Name / Synonyms	MVP/LRP
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

LRP antibody, VAULT1 antibody, MVP antibody, lung resistance-related protein antibody, major vault protein antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

99 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 158 and 380 of MVP/LRP (Uniprot ID# Q14764)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MVP/LRP(107-11081) antibody at 1:200 dilution.

MVP/LRP antibody detects MVP/LRP protein at cytosol on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
MVP/LRP antibody (107-11081) dilution: 1:500.

MVP/LRP antibody detects MVP protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg BCL-1 whole cell lysate/extract
E. 30 µg Raw264.7 whole cell lysate/extract
7.5 % SDS-PAGE
MVP/LRP antibody (107-11081) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LRP(MVP)(107-11081) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
107-11081 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products