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Anti-MYO1C Antibody
Catalog #: 144-06936
MYO1C Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00159 |
| Gene Id | 4641 |
| Gene Symbols | MYO1C |
| Protein Name / Synonyms | MYO1C, MMI-beta, MMIb, NMI, myr2, myosin IC |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:100
IHC 1:50 - 1:200
IF 1:50 - 1:100
Format
Calculated MW
117kDa/119kDa/121kDa
Observed MW
122kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 769-1028 of human MYO1C (NP_203693.3).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using MYO1C antibody (144-06936) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody (144-06936) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver injury using MYO1C antibody (144-06936) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody (144-06936) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody (144-06936) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody (144-06936) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using MYO1C antibody (144-06936).
Expiration:
12 months from the date of shipment when stored properly.