3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-MyoD1 (Center) Antibody
Catalog #: 102-11672
Rabbit Anti-Human MyoD1 (Center) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P15172 |
| Gene Id | 4654 |
| Gene Symbols | MYOD1 |
| Protein Name / Synonyms | MyoD1 (Center) |
| Clonality | Polyclonal |
| Preparation | This MyoD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-240 amino acids from the Central region of human MyoD1. |
| Concentration (lot specific) | 0.470 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis.
Specificity
Other Names
MYOD1; BHLHC1; MYF3; MYOD; Myoblast determination protein 1; Class C basic helix-loop-helix protein 1; Myogenic factor 3
NCBI Accession #
NP_002469.2
Other Accession #
NP_002469.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
34501
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | MyoD1 Antibody (Center) (Cat# 102-11672) western blot analysis in Hela cell line and human placenta tissue lysates (35ug/lane).This demonstrates the MyoD1 antibody detected the MyoD1 protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with MyoD1 Antibody (Center)(Cat# 102-11672). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MyoD1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MyoD1 immunoreactivity is localized to nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.