Anti-MyoGEF (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q3KR16
Gene Id	55200
Gene Symbols	PLEKHG6
Protein Name / Synonyms	MyoGEF (N-term)
Clonality	Polyclonal
Preparation	This MyoGEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-87 amino acids from the N-terminal region of human MyoGEF.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Guanine nucleotide exchange factor activating the small GTPase RHOA, which, in turn, induces myosin filament formation. It Also activates RHOG and does not activate RAC1, or to a much lower extent than RHOA and RHOG. Its part of a functional unit, involving PLEKHG6, MYH10 and RHOA, at the cleavage furrow to advance furrow ingression during cytokinesis. In epithelial cells, It is required for the formation of microvilli and membrane ruffles on the apical pole. Along with EZR, it is required for normal macropinocytosis.

Specificity

Other Names

PLEKHG6; Pleckstrin homology domain-containing family G member 6; Myosin-interacting guanine nucleotide exchange factor

NCBI Accession #

NP_001138328.1;NP_001138329.1;NP_060643.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

88960

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of MyoGEF Antibody (N-term) (Cat# 102-17017) in NCI-H460 cell line lysates (35ug/lane). MyoGEF (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma with MyoGEF Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of NCI-H292 cells using MyoGEF Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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