Anti-NANOG (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q9H9S0
Gene Id	79923
Gene Symbols	NANOG
Protein Name / Synonyms	NANOG (N-term)
Clonality	Polyclonal
Preparation	This NANOG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-49 amino acids from the N-terminal region of human NANOG.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.

Specificity

Predicted Reactivity

Monkey

Other Names

NANOG; Homeobox protein NANOG; Homeobox transcription factor Nanog

NCBI Accession #

NP_079141.2

Other Accession #

Q6NSW7; Q5TM84

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

34620

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of anti-NANOG Antibody (N-term) (Cat# 102-12697) in K562 cell line lysates (35ug/lane). NANOG (arrow) was detected using the purified Pab.Western blot analysis of NANOG (arrow) using rabbit polyclonal NANOG Antibody (N-term) (Cat# 102-12697). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NANOG gene (Lane 2) (Origene Technologies).
	Fluorescent confocal image of SY5Y cells stained with 102-12697 NANOG (N-term) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with 102-12697 NANOG (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (5.25 µM, 25 min). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 3 min). Nanog immunoreactivity is localized mainly to the nuclei of the SY5Y cells.
	Formalin-fixed and paraffin-embedded human Spleen tissue reacted with NANOG Antibody (N-term)(Cat# 102-12697), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	NANOG Antibody (N-term) (Cat# 102-12697) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term)(Cat# 102-12697).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.