Synonyms
SAS antibody, NANS antibody, sialic acid synthase antibody, N-acetylneuraminate synthase antibody, N-acetylneuraminic acid phosphate synthase antibody, N-acetylneuraminate-9-phosphate synthase antibody, sialic acid phosphate synthase antibody, N-acetylneuraminic acid synthase antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 54 and 359 of NANS (Uniprot ID# Q9NR45)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded normal gastric epithelium (gland), using NANS(107-10519) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
NANS antibody
107-10519 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.