Anti-NCF4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q15080
Gene Id	4689
Gene Symbols	NCF4
Protein Name / Synonyms	NCF4 (C-term)
Clonality	Polyclonal
Preparation	This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the C-terminal region of human NCF4.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.

Specificity

Other Names

NCF4; SH3PXD4; Neutrophil cytosol factor 4; Neutrophil NADPH oxidase factor 4; SH3 and PX domain-containing protein 4; p40-phox

NCBI Accession #

NP_000622.2;NP_038202.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

39032

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of NCF4 antibody (C-term)(Cat# 102-17465) in K562 cell line lysates (35ug/lane). NCF4 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	NCF4 Antibody (C-term) (Cat# 102-17465) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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