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Anti-NCK1 (N-term) Antibody
Catalog #: 102-12124
Rabbit Anti-Human NCK1 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P16333 |
| Gene Id | 4690 |
| Gene Symbols | NCK1 |
| Protein Name / Synonyms | NCK1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 amino acids from the N-terminal region of human NCK1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Specificity
Other Names
NCK1; NCK; Cytoplasmic protein NCK1; NCK adaptor protein 1; SH2/SH3 adaptor protein NCK-alpha
NCBI Accession #
NP_001177725.1;NP_006144.1
Other Accession #
NP_006144.1; NP_001177725.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
42864
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | NCK1 Antibody (N-term) (Cat# 102-12124) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the NCK1 antibody detected the NCK1 protein (arrow). |
 | Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) (Cat# 102-12124). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCK1 gene. |
 | NCK1 Antibody (N-term) (Cat# 102-12124)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCK1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.