Anti-NCL (Center E443) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P19338
Gene Id	4691
Gene Symbols	NCL
Protein Name / Synonyms	NCL (Center E443)
Clonality	Polyclonal
Preparation	This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 428-455 amino acids from the Central region of human NCL.
Concentration (lot specific)	0.310 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Specificity

Predicted Reactivity

Monkey

Other Names

NCL; Nucleolin; Protein C23

NCBI Accession #

NP_005372.2

Other Accession #

Q4R4J7; NP_005372.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

76614

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	NCL Antibody (Center E443) (Cat# 102-11201) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).
	Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443) (Cat# 102-11201). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.
	NCL Antibody (Center E443) (Cat# 102-11201)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443)(Cat# 102-11201). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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