Rabbit Anti-Human NCL (Center P291) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P19338 |
| Gene Id | 4691 |
| Gene Symbols | NCL |
| Protein Name / Synonyms | NCL (Center P291) |
| Clonality | Polyclonal |
| Preparation | This NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human NCL. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Specificity
Predicted Reactivity
Monkey
Other Names
NCL; Nucleolin; Protein C23
NCBI Accession #
NP_005372.2
Other Accession #
Q4R4J7; NP_005372.2
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
76614
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | NCL Antibody (Center P291) (Cat# 102-12576) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow). |
 | Fluorescent confocal image of U251 cell stained with NCL Antibody (Center P291)(Cat# 102-12576).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NCL immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.