Anti-NDRG1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q92597
Gene Id	10397
Gene Symbols	NDRG1
Protein Name / Synonyms	NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, protein NDRG1
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW

33kDa/35kDa/42kDa

Observed MW

50kDa

Immunogen

A synthetic phosphorylated peptide around T346 of human NDRG1 (NP_001128714.1).

Modification

Phospho T346

Storage

-20°C

Images

Western blot analysis of extracts of Jurkat cells, using Phospho-NDRG1-T346 antibody (144-61522) at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - Phospho-NDRG1-T346 antibody (144-61522)

Expiration:

12 months from the date of shipment when stored properly.

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