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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P52848 |
| Gene Id | 3340 |
| Gene Symbols | NDST1 |
| Protein Name / Synonyms | NDST1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This NDST1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 820-849 amino acids from the C-terminal region of human NDST1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NDST1 is an essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
NDST1; HSST; HSST1; Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; N-heparan sulfate sulfotransferase 1; [Heparan sulfate]-glucosamine N-sulfotransferase 1; Heparan sulfate N-deacetylase 1; Heparan sulfate N-sulfotransferase 1
NCBI Accession #
NP_001534.1
Other Accession #
Q02353; Q3UHN9; NP_001534.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
100868
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50
 | NDST1 Antibody (C-term) (Cat# 102-11920) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the NDST1 antibody detected the NDST1 protein (arrow). |
 | NDST1 Antibody (C-term) (Cat# 102-11920)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NDST1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.