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Anti-NDUFA10 Antibody
Catalog #: 107-11129
NDUFA10 antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4705 |
| Protein Name / Synonyms | NDUFA10 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CI-42KD antibody, CI-42k antibody, FLJ21923 antibody, MGC5103 antibody, NDUFA10 antibody, complex I 42kDa subunit antibody, "NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial antibody", NADH-ubiquinone oxidoreductase 42 kDa subunit antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 10, 42kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
41 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 115 and 355 of NDUFA10 (Uniprot ID# O95299)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using NDUFA10(107-11129) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Breast ca, using NDUFA10(107-11129) antibody at 1:250 dilution.
NDUFA10 antibody detects NDUFA10 protein by Western blot analysis.
A. 50 µg mouse kidney lysate/extract
10% SDS-PAGE
NDUFA10 antibody (107-11129) dilution: 1:1000
A. 50 µg mouse kidney lysate/extract
10% SDS-PAGE
NDUFA10 antibody (107-11129) dilution: 1:1000
Sample (30 ug of whole cell lysate)
A: JurKat
10% SDS PAGE
107-11129 diluted at 1:1000
A: JurKat
10% SDS PAGE
107-11129 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.