NDUFA5 antibody [N1C3], 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4698 |
| Protein Name / Synonyms | NDUFA5 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
B13 antibody, CI-13KD-B antibody, CI-13kB antibody, DKFZp781K1356 antibody, FLJ12147 antibody, NUFM antibody, UQOR13 antibody, NDUFA5 antibody, type I dehydrogenase antibody, NADH-ubiquinone oxidoreductase 13 kDa-B subunit antibody, Complex I-13KD-B antibody, ubiquinone reductase antibody, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 antibody, complex I subunit B13 antibody, complex I 13kDa subunit B antibody, "NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
13 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 116 of NDUFA5 (Uniprot ID# Q16718)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NDUFA5(107-10945) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
107-10945 diluted at 1:1000
A: mouse brain
15% SDS PAGE
107-10945 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Hep G2
15% SDS PAGE
107-10945 diluted at 1:1000
A: Hep G2
15% SDS PAGE
107-10945 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.