Anti-NDUFB5 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	4711
Protein Name / Synonyms	NDUFB5
Concentration (lot specific)	0.44 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CISGDH antibody, DKFZp686N02262 antibody, FLJ30597 antibody, MGC111204 antibody, MGC12314 antibody, SGDH antibody, NDUFB5 antibody, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial antibody, complex I-SGDH antibody, NADH-ubiquinone oxidoreductase SGDH subunit antibody, complex I SGDH subunit antibody, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5, 16kDa antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

22 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 126 and 189 of NDUFB5 (Uniprot ID# O43674)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using NDUFB5(107-10990) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using NDUFB5(107-10990) antibody at 1:500 dilution.

NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A.30 µg 293T whole cell lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000

NDUFB5 antibody detects NDUFB5 protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
12 % SDS-PAGE
NDUFB5 antibody (107-10990) dilution: 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

Featured Products