Synonyms
B22 antibody, DKFZp566O173 antibody, FLJ22885 antibody, LYRM3 antibody, UQOR22 antibody, NDUFB9 antibody, CI-B22 antibody, NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 antibody, NADH-ubiquinone oxidoreductase B22 subunit antibody, complex I B22 subunit antibody, complex I-B22 antibody, LYR motif-containing protein 3 antibody, NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
22 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 162 of NDUFB9
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

NDUFB9 antibody detects NDUFB9 protein at cytosol on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma.
NDUFB9 antibody (107-10535) dilution: 1:500.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10535 diluted at 1:1000

Sample(30 ug whole cell lysate)
A: HeLa S3
B: Hep G2
12% SDS PAGE
107-10535 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.