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Anti-NDUFS1 Antibody
Catalog #: 107-11092
NDUFS1 antibody, 0.84 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 4719 |
| Protein Name / Synonyms | NDUFS1 |
| Concentration (lot specific) | 0.84 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
CI-75Kd antibody, CI-75k antibody, MGC26839 antibody, PRO1304 antibody, NDUFS1 antibody, "NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial antibody", mitochondrial NADH-ubiquinone oxidoreductase 75 kDa subunit antibody, complex I 75kDa subunit antibody, "complex I, mitochondrial respiratory chain, 75-kD subunit antibody", "NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase) antibody"
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
79 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 432 and 664 of NDUFS1 (Uniprot ID# P28331)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed A431, using NDUFS1(107-11092) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS1(107-11092) antibody at 1:250 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11092 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-11092 diluted at 1:1000
Sample (50 ug of whole cell lysate)
A: Rat brain
12% SDS PAGE
107-11092 diluted at 1:1000
A: Rat brain
12% SDS PAGE
107-11092 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: Molt-4
12% SDS PAGE
107-11092 diluted at 1:1000
A: Molt-4
12% SDS PAGE
107-11092 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.