NDUFS2 antibody, 0.89 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 4720 |
| Protein Name / Synonyms | NDUFS2 |
| Concentration (lot specific) | 0.89 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
CI-49 antibody, NDUFS2 antibody, complex 1, mitochondrial respiratory chain, 49-KD subunit antibody, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial antibody, NADH-ubiquinone oxidoreductase 49 kDa subunit antibody, complex I 49kDa subunit antibody, complex I-49kD antibody, NADH-ubiquinone oxidoreductase NDUFS2 subunit antibody, CI-49kD antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
53 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 200 and 430 of NDUFS2 (Uniprot ID# O75306)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
NDUFS2 antibody detects NDUFS2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: NDUFS2 protein stained by NDUFS2 antibody (107-11142) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37? for 30 min.
Green: NDUFS2 protein stained by NDUFS2 antibody (107-11142) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS2(107-11142) antibody at 1:250 dilution.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-11142 diluted at 1:10000
A: Mouse brain
10% SDS PAGE
107-11142 diluted at 1:10000
Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
107-11142 diluted at 1:1000
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
107-11142 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
