Synonyms
CI-51K antibody, CI51KD antibody, FLJ59059 antibody, UQOR1 antibody, NDUFV1 antibody, complex I 51kDa subunit antibody, mitochondrial NADH dehydrogenase ubiquinone flavoprotein 1 antibody, "NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial antibody", complex I 51 kda subunit antibody, mitochondrial NADH:ubiquinone oxidoreductase 51 kda subunit antibody, NADH-ubiquinone oxidoreductase 51 kDa subunit antibody, "complex I, mitochondrial respiratory chain antibody", "NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
51 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 218 and 459 of NDUFV1 (Uniprot ID# P49821)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using NDUFV1(107-10325) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10325 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hep G2
10% SDS PAGE
107-10325 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.