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Anti-NEK4 (internal) Antibody
Catalog #: 107-10351
NEK4 antibody [N2C1], Internal, 0.37 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 6787 |
| Protein Name / Synonyms | NEK4, Internal |
| Concentration (lot specific) | 0.37 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
MGC33171 antibody, NRK2 antibody, STK2 antibody, pp12301 antibody, NEK4 antibody, serine/threonine protein kinase-2 antibody, serine/threonine-protein kinase Nek4 antibody, serine/threonine-protein kinase 2 antibody, nimA-related protein kinase 4 antibody, serine/threonine-protein kinase NRK2 antibody, never in mitosis A-related kinase 4 antibody, serine/threonine kinase 2 antibody, NIMA (never in mitosis gene a)-related kinase 4 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
95 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 241 and 504 of NEK4 (Uniprot ID# P51957)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (107-10351) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (107-10351) diluted at 1:500.
Blue: Hoechst 33343 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(107-10351) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: A431
B: H1299
7.5% SDS PAGE
107-10351 diluted at 1:1000
A: A431
B: H1299
7.5% SDS PAGE
107-10351 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.