Anti-Neprilysin (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P08473
Gene Id	4311
Gene Symbols	MME
Protein Name / Synonyms	Neprilysin (C-term)
Clonality	Polyclonal
Preparation	This Neprilysin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human Neprilysin.
Concentration (lot specific)	0.400 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.

Specificity

Predicted Reactivity

Mouse, Rat, Rabbit

Other Names

MME; EPN; Neprilysin; Atriopeptidase; Common acute lymphocytic leukemia antigen; Enkephalinase; Neutral endopeptidase 24.11; Skin fibroblast elastase; CD_antigen=CD10

NCBI Accession #

NP_000893.2;NP_009218.2;NP_009219.2;NP_009220.2

Other Accession #

P07861; P08049; Q61391

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

85514

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of Neprilysin Antibody (C-term) (Cat# 102-17280) in A2058,A375,Ramos cell line lysates (35ug/lane). EPN (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human kidney carcinoma with Neprilysin Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Neprilysin Antibody (C-term) (Cat# 102-17280) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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