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Anti-Nesfatin Antibody
Catalog #: RB-08-0022
Rabbit Anti-Human Nesfatin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | NP_067695 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide (N-terminus) derived from Human nesfatin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbitt serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CTALEAVPIDVDKTK |
| Recommended Applications | ELISA (recommended work dilution= 1:10e6(at least detecting 10ng/ml)), Western Blotting (recommended work dilution= 1:1000-5000(at least)) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the data of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 ?g/ml. Please avoid freeze-thaw cycles. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Nesfatin-1, derived from the protein NEFA/nucleobindin2 (NUCB2), is a newly identified peptide that acts as a potent satiety agent. It is an 82 amino acid peptide that suppresses food intake after intracerebroventricular injection. Nesfatin-1 and its precursor NUCB2 were identified by subtraction cloning in cell lines of both neuronal and adipocytic origin. It has been reported that peptides involved in the regulation of ingestive behavior are also involved in the regulation of the stress response. It is located in the hypothalamic nuclei, including the paraventricular nucleus (PVN) and supraoptic nucleus (SON). The conversion of NUCB2 into Nesfatin-1 is indispensable for its activity in vivo. Nesfatin-1 is found in discrete nuclei of the hypothalamus where it probably activates a leptin-independent melanocortin pathway. Nesfatin-1 crosses the Blood Brain Barrier (BBB) in both the blood-to-brain and brain-to-blood directions by a nonsaturable system. NUCB2 is also expressed in the adipocyte cell line 3T3L1 suggesting other functions of Nesfatin-1 outside brain or peripheral source of Nesfatin-1 affecting brain function.
Specificity
The rabbit anti-human Nesfatin antibody detects specifically target derived from human recombinant protein as well as KLH-conjugated synthetic peptide as immunogen. Cross reactivity with mouse and rat are expected from sequence similarity.
Storage
Store at -20°C, if not intended for use within a month, Keep it at 4°C and minimize freezing and thawing when use.
Related Products
Nesfatin Immunodetection Kit (Western-blot-based, cat#RB-08-0008K) ; Nesfatin Immunoassay Kit (EIA, cat# EIA-Nes-1) ; Obesity antibody arrays(cat#AAH-OBS-1-1)
References
- Price CJ, et.al. Nesfatin-1 inhibits NPY neurons in the arcuate nucleus. Brain Res. 2008. Oh-I S, et.al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature. 2006.
- Brailoiu GC, et.al. Nesfatin-1: distribution and interaction with a G protein-coupled receptor in the rat brain. Endocrinology. 2007.
Expiration:
12 months from the date of shipment when stored properly.