Anti-Neurogenin3 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y4Z2
Gene Id	50674
Gene Symbols	NEUROG3
Protein Name / Synonyms	Neurogenin3 (N-term)
Clonality	Polyclonal
Preparation	This Neurogenin3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human Neurogenin3.
Concentration (lot specific)	1.900 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Neuroscience
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Neurogenin-3 belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm. Neurogenin-3 also required for the specification of a common precursor of the 4 pancreatic endocrine cell types. Defects in NEUROG3 are the cause of congenital malabsorptive diarrhea 4 (DIAR4). DIAR4 is an autosomal recessive disorder characterized by generalized malabsorption and a paucity of enteroendocrine cells.

Specificity

Other Names

NEUROG3; ATOH5; BHLHA7; NGN3; Neurogenin-3; Class A basic helix-loop-helix protein 7; Protein atonal homolog 5

NCBI Accession #

NP_066279.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

23077

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	The Neurogenin3 (N-term) polyclonal antibody (Cat# 102-14610) is used in Western blot to detect Neurogenin3 in mouse liver tissue lysate.
	Neurogenin3 Antibody (N-term) (Cat# 102-14610) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Neurogenin3 antibody detected the Neurogenin3 protein (arrow).
	Western blot analysis of Neurogenin3 (arrow) using rabbit polyclonal Neurogenin3 Antibody (N-term) (Cat# 102-14610). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Neurogenin3 gene.
	Neurogenin3 Antibody (N-term) (Cat# 102-14610) immunohistochemistry analysis in formalin fixed and paraffin embedded human large intestine followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Neurogenin3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of Neurogenin3 Antibody (N-term) (Cat# 102-14610) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).