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Anti-Neuropeptide B Antibody, Biotinylated
Catalog #: 130-00014B-50
Rabbit anti-Neuropeptide B Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8NG41 |
| Gene Id | 256933 |
| Gene Symbols | NPB PPL7 PPNPB |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography |
| Immunogen Sequence | CYKPAAGPHHYSVGRASGLLS |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Neuropeptide B (NPB) is an endogenous peptide ligand for G protein-coupled receptor-7 (GPR7; MIM 600730). It may be involved in the regulation of feeding, neuroendocrine system, memory, learning and in the afferent pain pathway, by similarity. The deduced 125-amino acid prepropeptide contains an Nterminal 24-amino acid secretory signal and 2 diarginine (dibasic) motifs that are potential peptideprocessing sites. In rat, high expression was detected in lymphoid organs, central nervous system (CNS), mammary gland, and uterus. In situ hybridization of rat brain detected expression in hypothalamus and hippocampus.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody specifically binds to human NBP. Cross reactivity with mouse and rat NPB was detected.
References
- Fujii, R. et al. (2002) "Identification of a neuropeptide modified with bromine as an endogenous ligand for GPR7." J. Biol. Chem. 277:34010-34016(2002)
- Brezillon, S. et al. "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8." J. Biol. Chem. 278:776-783(2003)
Expiration:
12 months from the date of shipment when stored properly.