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Anti-Neuropeptide W Antibody
Catalog #: 130-00012
Rabbit Anti-Human Neuropeptide W Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Specificity | This antibody can specifically bind to human Neuropeptide W. Cross reactivity with mouse and rat NPW was detected. |
| Accession Number | Q8N729 |
| Gene Id | 283869 |
| Gene Symbols | NPW PPL8 PPNPW |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography |
| Immunogen Sequence | CWYKHVASPRYHTVGRAAG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000) |
| Reconstitution | Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Neuropeptide W is an endogenous peptide ligand for GPR8, a G protein-coupled receptor. This protein is detected at high levels in the substantia nigra, fetal kidney and trachea; at lower levels in testis, uterus, ovary and placenta, and not detectable in many regions of the central nervous system. It is also detected at high levels in lymphoblastic leukemia and colorectal adenocarcinoma. The product of this gene is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade.
Typical Data
Storage/Stability
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
Expiration
12 months from the date of shipment when stored properly.
References
- Shimomura, Y. et al. (2002) "Identification of neuropeptide W as the endogenous ligand for orphan Gprotein-coupled receptors GPR7 and GPR8." J. Biol. Chem. 277:35826-35832(2002)
- Brezillon, S. et al. (2003) "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8." J. Biol. Chem. 278:776-783(2003)