3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-Neuropeptide W Antibody, Biotinylated
Catalog #: 130-00012B-50
Rabbit anti-Neuropeptide W Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q8N729 |
| Gene Id | 283869 |
| Gene Symbols | NPW PPL8 PPNPW |
| Clonality | Polyclonal |
| Immunogen | The immunogen was a synthetic peptide. This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography |
| Immunogen Sequence | CWYKHVASPRYHTVGRAAG |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 64, 000), Western Blotting (recommended work dilution= 1:1, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Research Area | Neuroscience |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Neuropeptide W is an endogenous peptide ligand for GPR8, a G protein-coupled receptor. This protein is detected at high levels in the substantia nigra, fetal kidney and trachea; at lower levels in testis, uterus, ovary and placenta, and not detectable in many regions of the central nervous system. It is also detected at high levels in lymphoblastic leukemia and colorectal adenocarcinoma. The product of this gene is processed into 23- and 30-amino acid neuropeptides that bind and activate two G-protein coupled receptors in the central nervous system. The neuropeptides have been shown to enhance cortisol secretion from adrenal cells through the adenylate cyclase/protein kinase A signaling cascade.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody can specifically bind to human Neuropeptide W. Cross reactivity with mouse and rat NPW was detected.
References
- Shimomura, Y. et al. (2002) "Identification of neuropeptide W as the endogenous ligand for orphan Gprotein-coupled receptors GPR7 and GPR8." J. Biol. Chem. 277:35826-35832(2002)
- Brezillon, S. et al. (2003) "Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8." J. Biol. Chem. 278:776-783(2003)
Expiration:
12 months from the date of shipment when stored properly.