Anti-NFATC4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q14934
Gene Id	4776
Gene Symbols	NFATC4
Protein Name / Synonyms	NFATC4 (C-term)
Clonality	Polyclonal
Preparation	This NFATC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 744-773 amino acids from the C-terminal region of human NFATC4.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq].

Specificity

Other Names

NFATC4; NFAT3; Nuclear factor of activated T-cells, cytoplasmic 4; T-cell transcription factor NFAT3

NCBI Accession #

NP_001129494.1;NP_001185894.1;NP_001185895.1;NP_001185896.1;NP_004545.2

Other Accession #

NP_004545.2; NP_001129494.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

95449

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

NFATC4 Antibody (C-term) (Cat# 102-11896) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the NFATC4 antibody detected the NFATC4 protein (arrow).

Fluorescent confocal image of U251 cell stained with NFATC4 Antibody (C-term)(Cat# 102-11896).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFATC4 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFATC4 immunoreactivity is localized to Cytoplasm significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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