Anti-NFKBIA ( S32/36 ) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P25963
Gene Id	4792
Gene Symbols	NFKBIA
Protein Name / Synonyms	NFKBIA ( S32/36 )
Clonality	Polyclonal
Preparation	This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from human NFKBIA.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC, IF
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).

Specificity

Predicted Reactivity

Mouse, Pig

Other Names

NFKBIA; IKBA; MAD3; NFKBI; NF-kappa-B inhibitor alpha; I-kappa-B-alpha; Major histocompatibility complex enhancer-binding protein MAD3

NCBI Accession #

NP_065390.1

Other Accession #

Q08353; Q9Z1E3

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

35609

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50; IF: 1:10-50

	Western blot analysis of NFKBIA-S32/36 (Cat# 102-17741) in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Confocal immunofluorescent analysis of NFKBIA Antibody ( S32/36 )(Cat# 102-17741) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products