Anti-NFYB (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P25208
Gene Id	4801
Gene Symbols	NFYB
Protein Name / Synonyms	NFYB (N-term)
Clonality	Polyclonal
Preparation	This NFYB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human NFYB.
Concentration (lot specific)	0.420 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken

Other Names

NFYB; HAP3; Nuclear transcription factor Y subunit beta; CAAT box DNA-binding protein subunit B; Nuclear transcription factor Y subunit B

NCBI Accession #

NP_006157.1

Other Accession #

P63140; P63139; P25207; Q32KW0; NP_006157.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22831

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

NFYB Antibody (N-term) (Cat# 102-12436) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the NFYB antibody detected the NFYB protein (arrow).

Fluorescent confocal image of Hela cell stained with NFYB Antibody (N-term)(Cat# 102-12436). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NFYB primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NFYB immunoreactivity is localized to nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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