Anti-NHP2-like protein 1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	4809
Protein Name / Synonyms	NHP2-like protein 1
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

15.5K antibody, FA-1 antibody, FA1 antibody, NHPX antibody, OTK27 antibody, SNRNP15-5 antibody, SNU13 antibody, SPAG12 antibody, SSFA1 antibody, NHP2L1 antibody, [U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein antibody, sperm specific antigen 1 antibody, high mobility group-like nuclear protein 2 homolog 1 antibody, U4/U6.U5 tri-snRNP 15.5 kDa protein antibody, small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5) antibody, NHP2-like protein 1 antibody, non-histone chromosome protein 2-like 1 antibody, NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

14 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 128 of NHP2-like protein 1 (Uniprot ID# P55769)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg GL261 whole cell lysate/extract
C. 30 µg C8D30 whole cell lysate/extract
D. 30 µg NIH-3T3 whole cell lysate/extract
E. 30 µg BCL-1 whole cell lysate/extract
F. 30 µg Raw264.7 whole cell lysate/extract
G. 30 µg C2C12 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (107-10826) dilution: 1:1000

NHP2L1 antibody detects NHP2L1 protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
15 % SDS-PAGE
NHP2L1 antibody (107-10826) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NHP2-like protein 1(107-10826) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using NHP2-like protein 1(107-10826) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: HeLa
B: Hep G2
12% SDS PAGE
107-10826 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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