Anti-NIP1 (BH3 Domain Specific) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q12981
Gene Id	662
Gene Symbols	BNIP1
Protein Name / Synonyms	NIP1 (BH3 Domain Specific)
Clonality	Polyclonal
Preparation	This NIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-127 amino acids from human NIP1.
Concentration (lot specific)	1.930 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	Apoptosis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NIP1 (BNIP1) is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four products of unknown function. Transcript variant BNIP1 contains the entire coding region of the gene. This variant contains a fully conserved BH3 domain, which has been associated with pro-apoptotic function.

Specificity

Other Names

BNIP1; NIP1; SEC20L; Vesicle transport protein SEC20; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; Transformation-related gene 8 protein

NCBI Accession #

NP_001196.2;NP_053581.2;NP_053582.2;NP_053583.2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

26132

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of anti-NIP1 BH3 Domain Pab (Cat# AP1335a) in HL60 cell lysates (35ug/lane). NIP1 BH3 Domain (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products