Anti-Nkx2.5 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	1482
Protein Name / Synonyms	Nkx2.5
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CHNG5 antibody, CSX antibody, CSX1 antibody, FLJ52202 antibody, FLJ97166 antibody, FLJ97195 antibody, FLJ97197 antibody, FLJ99536 antibody, NKX2.5 antibody, NKX2E antibody, NKX4-1 antibody, NKX2-5 antibody, tinman paralog antibody, homeobox protein Nkx-2.5 antibody, cardiac-specific homeobox 1 antibody, NK2 transcription factor related, locus 5 antibody, homeobox protein NK-2 homolog E antibody, homeobox protein CSX antibody, NK2 transcription factor related, locus 5 (Drosophila) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

35 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 218 of Nkx2.5 (Uniprot ID# P52952)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using Nkx2.5(107-10552) antibody at 1:200 dilution.

Sample (50 ug of whole cell lysate)
A: mouse heart
10% SDS PAGE
107-10552 diluted at 1:1000

Nkx2.5 antibody detects NKX2-5 protein by Western blot analysis.
A. 50 µg rat heart lysate/extract
10 % SDS-PAGE
Nkx2.5 antibody (107-10552) dilution: 1:500

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NKX2-5(107-10552) antibody at 1:200 dilution.

Sample(30 ug whole cell lysate)
A:H1299
B:Raji
10% SDS PAGE
107-10552 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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