Rabbit Anti-Human NKX3-1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q99801 |
| Gene Id | 4824 |
| Gene Symbols | NKX3-1 |
| Protein Name / Synonyms | NKX3-1 (Center) |
| Clonality | Polyclonal |
| Preparation | This NKX3-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-145 amino acids from the Central region of human NKX3-1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cardiovascular Disease, Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
NKX3-1 is a transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. It play an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. It Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Specificity
Predicted Reactivity
Mouse
Other Names
NKX3-1; NKX3.1; NKX3A; Homeobox protein Nkx-3.1; Homeobox protein NK-3 homolog A
NCBI Accession #
NP_001243268.1;NP_006158.2
Other Accession #
P97436
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
26350
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of NKX3-1 Antibody (Center) (Cat# 102-18110) in MCF-7 cell line lysates (35ug/lane). NKX3-1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with NKX3-1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | NKX3-1 Antibody (Center) (Cat# 102-18110) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.