Anti-NME2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	P22392
Gene Id	654364
Gene Symbols	NME2
Protein Name / Synonyms	NME2 (N-term)
Clonality	Polyclonal
Preparation	This NME2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human NME2.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NM2 is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. NEM2 has been identified as a putative tumor suppressor. High expression of mouse NME2 is detected in heart, liver, and kidney, with moderate expression in skeletal muscle, and negligible expression in other mouse tissues examined.

Specificity

Other Names

NME2; NM23B; Nucleoside diphosphate kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2

NCBI Accession #

NP_001018146.1;NP_001018147.1;NP_001018148.1;NP_001018149.1;NP_002503.1

Other Accession #

P19804; Q01768

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

17298

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50

	NME2 Antibody (F40) (Cat# 102-17790) western blot analysis in HL-60,Jurkat,MCF-7 cell line,mouse kidney and rat stomach tissue lysates (35ug/lane).This demonstrates the NME2 antibody detected the NME2 protein (arrow).
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Fluorescent confocal image of Hela cell stained with NME2 Antibody (N-term)(Cat# 102-17790).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NME2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). NME2 immunoreactivity is localized to Cytoplasm and Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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