Anti-NOD1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y239
Gene Id	10392
Gene Symbols	NOD1
Protein Name / Synonyms	NOD1 (C-term)
Clonality	Polyclonal
Preparation	This NOD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 923-951 amino acids from the C-terminal region of human NOD1.
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene encodes a member of the NOD (nucleotide-binding oligomerization domain) family. This member is a cytosolic protein. It contains an N-terminal caspase recruitment domain (CARD), a centrally located nucleotide-binding domain (NBD), and 10 tandem leucine-rich repeats (LRRs) in its C terminus. The CARD is involved in apoptotic signaling, LRRs participate in protein-protein interactions, and mutations in the NBD may affect the process of oligomerization and subsequent function of the LRR domain. This protein is an intracellular pattern-recognition receptor (PRR) that initiates inflammation in response to a subset of bacteria through the detection of bacterial diaminopimelic acid. Multiple alternatively spliced transcript variants differring in the 5' UTR have been described, but the full-length nature of these variants has not been determined.

Specificity

Other Names

NOD1; CARD4; Nucleotide-binding oligomerization domain-containing protein 1; Caspase recruitment domain-containing protein 4

NCBI Accession #

NP_006083.1

Other Accession #

NP_006083.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

107691

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	NOD1 Antibody (C-term) (Cat# 102-11264) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the NOD1 antibody detected the NOD1 protein (arrow).
	NOD1 Antibody (C-term) (Cat# 102-11264)immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NOD1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	NOD1 Antibody (C-term) (Cat# 102-11264) western blot analysis in HL-60,CEM cell line lysates (35ug/lane).This demonstrates the NOD1 antibody detected the NOD1 protein (arrow).
	Confocal immunofluorescent analysis of NOD1 Antibody (C-term)(Cat# 102-11264) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	NOD1 Antibody (C-term) (Cat# 102-11264) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.