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Anti-NOS3 (N-term) Antibody
Catalog #: 102-17040
Rabbit Anti-Human NOS3 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P29474 |
| Gene Id | 4846 |
| Gene Symbols | NOS3 |
| Protein Name / Synonyms | NOS3 (N-term) |
| Clonality | Polyclonal |
| Preparation | This NOS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-75 amino acids from the N-terminal region of human NOS3. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases.
Specificity
Predicted Reactivity
Bovine, Pig
Other Names
NOS3; Nitric oxide synthase, endothelial; Constitutive NOS; EC-NOS; Endothelial NOS; NOS type III
NCBI Accession #
NP_000594.2;NP_001153581.1;NP_001153582.1;NP_001153583.1
Other Accession #
Q28969; P29473
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
133289
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50
 | Western blot analysis of NOS3 Antibody (N-term) (Cat# 102-17040) in HL-60 cell line lysates (35ug/lane). NOS3 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with NOS3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Confocal immunofluorescent analysis of NOS3 Antibody (N-term)(Cat# 102-17040) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue). |
 | NOS3 Antibody (N-term) (Cat# 102-17040) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.