Phospho-NPM1-T199 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P06748 |
| Gene Id | 4869 |
| Gene Symbols | NPM1 |
| Protein Name / Synonyms | NPM1, B23, NPM, nucleophosmin |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a phosphoprotein which moves between the nucleus and the cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
28kDa/29kDa/32kDa
Observed MW
38kDa
Immunogen
A synthetic phosphorylated peptide around T199 of human NPM1 (NP_001032827.1).
Modification
Phospho T199
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-NPM1-T199 antibody (144-61545) at 1:1000 dilution. Hela cells were treated by Nocodazole (100 ng/ml) for 24 hours. PC-12 cells were treated by Nocodazole (1 ?g/ml) for 16 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Expiration:
12 months from the date of shipment when stored properly.