Anti-NPM1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P06748
Gene Id	4869
Gene Symbols	NPM1
Protein Name / Synonyms	NPM1 (C-term)
Clonality	Polyclonal
Preparation	This NPM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 198-226 amino acids from the C-terminal region of human NPM1.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, FC, IHC-P, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF. NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia.

Specificity

Other Names

NPM1; NPM; Nucleophosmin; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin

NCBI Accession #

NP_001032827.1;NP_002511.1;NP_954654.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

32575

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; FC: 1:10-50; IHC: 1:10-50

	Fluorescent image of A549 cell stained with NPM1 Antibody (C-term)(Cat# 102-15171/SH090717BE).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NPM1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).NPM1 immunoreactivity is localized to Nucleus and Nucleolus significantly.
	NPM1 Antibody (C-term) (Cat# 102-15171) western blot analysis in Hela,Jurkat cell line lysates (35ug/lane).This demonstrates the NPM1 antibody detected the NPM1 protein (arrow).
	NPM1 Antibody (C-term) (Cat# 102-15171) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	NPM1 Antibody (C-term) (102-15171)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NPM1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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